Today in lab we looked at our test tube and plate from Tuesday to see what amount of oxygen our unknown needs to grow. From the test tube we were able to tell that it seemed to be obligate aerobic because all the grow was at the top of the liquid.
It looked like a jellyfish to me (Courtney). But when we looked at the bottom of the test tube there was a very small amount of growth and when we looked at the plate there was one single colony of growth. This shows that our bacteria is actually facultative but only slightly.
We also compared our unknown to other groups and with Theresa and Lisa who shared the plate with us. Their bacteria was truly facultative.
The next couple experiments we did were also grouped with Lisa and Theresa to save plates and agar. We needed to see which nutrients our bacteria uses to grow. The first test was to see the use of starch. For this we used a starch agar plate, which was a transparent color, with our bacteria on one side and their's on the other. The second was the same set up but on a skim milk agar plate that looked slightly yellow-orange and the third was on a tributyrin plate that was a cool blue color. The starch plate will show starch hydrolysis, the skim milk will show casein hydrolysis, and the tributyrin will show triglyceride or fat hydrolysis.
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