7 Test Tubes & 1 Positive Reaction

On Wednesday we needed to go into lab and document the reactions after 24 hours. There wasn't much change from Tuesday.

        lactose                                                         sucrose

       glucose                                                       comparison of all 3 sugars

       litmus milk                                                 MR-VP before adding reagents

      gelatin                                                          triple sugar iron

Today in lab we checked the 48 hour reactions and added reagents to the MR-VP experiment.
The three different sugar tests, lactose, sucrose and glucose, were negative meaning that our bacteria doesn't use carbohydrates to grow. The TSI test also showed the same result of negative.

The litmus milk test still showed no change. The gelatin test showed a small growth and a liquid portion at the top of the tube. This means that our bacteria is able to hydrolyze gelatin. For the MR-VP test we needed to add reagents "A" and "B" to 1mL and methyl red to the rest of the test tube. This made the test show a negative result.

      positive gelatin                                             "A"

     MR-VP with "A" & "B"                            Swirling for 5 minutes.....

   MR-VP with methyl red