Thursday, November 10, 2011

What is he doing with that glove??

Today in lab we finished the experiments we started on Tuesday.
Indole - After we added Kovak's reagent the top layer turned yellow which means tryptophan was not hydrolyzed.

Citrate - When we looked at the test tube the top of the agar had turned blue which means it is positive for using citrate.

Urea - The color slightly changed but we left it in the incubator to see if it is actually a positive result.


Nitrate - We added 5 drops of reagent A and 5 drops of Reagent B and mixed the broth. Ours made red bubbles and when we mixed it more it turned to a pink color. This is a positive reaction meaning nitrite ions were present.

Oxidase - We swabbed our unknown then dripped oxidase onto the swab. It did not change color so it was a negative result.

 

Antibiotics - Our plate showed that Tetracycline slightly worked, clearing 1.3cm which means it is not sensitive, Vancomycin cleared 1.8cm which means it is not sensitive, Chloramphenol cleared 2.0cm which means it is sensitive and Neomycin cleared 1.8cm which means it is also sensitive.


While we were doing our experiments, Dr. Pathakamuri was playing with a glove and made MRSA.


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